. Advanced Osmometer Model 3D3 ® Advanced Cryoscope Model 4D3 ® Service Manual 4D35SM Rev25 082714. Calibration.20 Cooling Assembly (3D3300/4D3300) 3D3/4D3 Circuit Description (Intel).21.4D3P300 Power Supply.

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PCB605 Assembly Drawing PCB605 Application Board Schematic 4D3605 Assembly Drawing PCB620 Assembly Drawing PCB620 Processor Board Schematic Interconnection Schematic - Intel APPENDICES A: Symbol Definitions B: Product Disposal and Recycling C: Service Log Index ® The Advanced 3D3/4D3 Service Manual. Model 3D3 Osmometer Specifications Electrical: Drift: Less than 1 digit per month Voltage: 100 to 130 VAC or 200 to 250 VAC Ambient Temp. 1 digit for every 5°C (9°F) Frequency: 50 or 60 Hz Effect: ambient temperature change. Fuses: 2-Amp time delay for 100-130 VAC. Operating Temperature (ambient): 18°C to 35°C (64°F to 95°F) Room Humidity: 5 to 80% R.H. (non-condensing) Drift: Less than 1 digit per month Ambient Temp. 1 digit for every 5°C (9°F) Effect: ambient temperature change.

Advanced Instruments Model 3250 Osmometer Manual The advanced osmometer ˆ0& 3250 vwr international, advanced ® osmometer model 3250 users guide service manual.

® The Advanced 3D3/4D3 Service Manual. 3D3 and the 4D3 are identical to one the problem. Use a telephone as close to the another. For clarity when replacing parts. Notes: ® The Advanced 3D3/4D3 Service Manual.

Route power cords so that they will not be This symbol indicates the presence walked on, tripped on, or pinched by items of alternating current (AC). Placed upon or against them. Pay particu- ® The Advanced 3D3/4D3 Service Manual. The power cord, extension cord, power strip or power input module is damaged. ® The Advanced 3D3/4D3 Service Manual. Equipment for Measurement,. Connections to this product must be made Control and Laboratory Use - with shielded cables.

Use of non-shielded General Requirements'; Including Amendment Two cables may violate RFI/EMI limits. ® The Advanced 3D3/4D3 Service Manual. Canadian and U.S.

Safety standards by Intertek Testing Services NA, Inc. The ETL mark is approved in the United States as a Nationally Recognized Testing Lab (NRTL) by OSHA, and in Canada by the Standards Council of Canada. ® The Advanced 3D3/4D3 Service Manual. General Overview. The larger board is called the application has made several improvements to the board, and the smaller board is called the 3D3/4D3 system. It is important to identify processor board. The processor board some of these changes to properly service contains an Intel 80C186 microprocessor your instrument.

Power Entry Module Q. RS-232C Port R. Printer Port S.

Printer Power Outlet T. Heat Transfer Return Tube J.

Supervisor/Operator Keyswitch U. Heat Transfer Inlet Tube K. Barcode Port ® The Advanced 3D3/4D3 Service Manual. Remove the keypad display connectors cryoscopes with suffix 'A' or later, and to from the keypad on the front cover.

All 3D3 osmometers: 5. Remove the two screws from the head 1.

Turn off the power and unplug the instru- cover (C) and lift off the head cover. Calibration The 3D3 has two ranges, each with its own calibration. To calibrate the low range, three Calibrate the instrument according to the freezing-point tests are required at the 100 instructions below: mOsm/kg level, and six tests are required at the 1500 mOsm/kg level.

When the Power Supply supercooled sample reaches a set temperature (3000 mOsm for the 3D3 in the low range The power entry assembly interfaces the AC and 5400 mOsm for the 3D3 in the high voltage presented to the instrument with the range. (1 The RTC uses an internal clock circuit with word = 2 bytes, 1 byte = 8 bits; therefore, 16 an internal, 10-year life, lithium battery to bits = 1 word). ® The Advanced 3D3/4D3 Service Manual. The application PCB contains all circuitry specific to the 3D3/4D3 instruments. The The thermoelectric cooler is controlled by the board includes voltage supplies, indicators, FET driver transistor mounted on the rear analog-to-digital converters, drive circuitry, panel (Q1B). Data Set Ready to 3D3/4D3 plies up to 1 Amp at 12 VDC via the follow- Request to Send from 3D3/4D3 ing connections: Clear to Send to 3D3/4D3 ® The Advanced 3D3/4D3 Service Manual. Operator position, the user cannot change 4D3 cryoscopes that have a suffix before setup or calibration settings.

'E' and 3D3 osmometers that have a suf- fix before 'C' do not have the head tran- Head Transition Board (M20450) Descrip- sition board. Notes: ® The Advanced 3D3/4D3 Service Manual. The power entry assembly interfaces the AC supercooled sample reaches a set temperature voltage presented to the instrument with the (3000 mOsm for the 3D3 in the low range transformer. The transformer provides 120 and 5400 mOsm for the 3D3 in the high VAC to the power supply board and the head range. The DB-9 RS-232 port conforms to the DTE head up and head down positions.

RS-232C standard and has the following pin assignments: Keypad Interface: A peripheral interface adapter is used to interface the keypad, ® The Advanced 3D3/4D3 Service Manual. Note that your instrument is only designed to 4D3 cryoscopes that have a suffix before support unidirectional communication with an 'E' and 3D3 osmometers that have a suf- external device. At this time, there is no pro- fix before 'C' do not have the head tran- tocol for bidirectional communication. The serial number suffix (referred in this manual simply as the suffix) indicates the revision of the instrument. The charts below list the major changes made at each revision of the 3D3 Osmometer and the 4D3 Cryoscope.

Model 3D3 Model 3D3. Added surge arrester to power December 1995 entry. Serial Suffix ‘K’ Cooling assembly change.

February 1996 Serial Suffix ‘L’ Painted underside of deck. December 1996 Serial Suffix ‘M’ Paint changed to off-white. May 1997 ® The Advanced 3D3/4D3 Service Manual.530 m°H Lactrol® 530 Reference 900 mOsm/kg Calibration Standard Solution (-512 m°C).3LA030 (10 5-mL ampules).3LA091 ® The Advanced 3D3/4D3 Service Manual. 9-Meter Shielded RS-232.330059 Thermal Printer with Interface Cable, Service Manual, 3D3/4D3.4D35SM Operation Manual, Thermal Paper Supervisor Keys.3D3185 Roll, and Printer Power Supply User’s Guide.

Notes: ® The Advanced 3D3/4D3 Service Manual. Cleaning and Maintenance The 3D3/4D3 cooling assembly should be 7. Insert an empty sample tube into the cleaned once every two months to insure opti- freezing chamber and press START.

Mal performance. Run this test for 10-15 minutes and then press STOP. Notes: ® The Advanced 3D3/4D3 Service Manual. Free police car sound effects.

Remove the white Delrin elevator and spring (C). (Save for replacement when 15. Reassemble the instrument by reversing cleaning is complete.) steps 1-8. ® The Advanced 3D3/4D3 Service Manual. 2-6 from the Osmometer Well Cleaning instruction. Add a new bottle of heat transfer fluid and prime the pump.

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The instrument is ready for testing. ® The Advanced 3D3/4D3 Service Manual. Introduction Problems with the performance of the Before you repair or replace any 3D3/4D3 are usually identified by the soft- parts, turn the instrument off and ware and displayed as error messages. The then back on after a short delay. 13mm (1/2”). If the freeze amplitude is not within the correct range, follow steps 4-5 below. Remove the head cover and clear away any metal chips between the coil frame and the clapper (A).

® The Advanced 3D3/4D3 Service Manual. Not acceptable. Replace stir wire driver TR1 on the head transition board. Order or use ferrite bead. Advanced Instruments part #91035 to get the appropriate ferrite material. Not acceptable. Replace stir wire driver Figure 1.

(F) is in the middle of the head down sensor (G).If you have a 4D3 cryoscope without a suffix, the head sensors are mounted directly to the bracket, and not to the head sensor board. ® The Advanced 3D3/4D3 Service Manual. The red LEDs marked. The gears (B) are turning properly.

“Head Up” and “Head Dn” indicate. The sensors (C) are detecting the head-up that the corresponding optical sensor and head-down conditions. Has been tripped. ® The Advanced 3D3/4D3 Service Manual. 2000 mOsm calibration stan- temperature.

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The probe begins to cool after dard for a 3D3/4D3 into the freezing the target temperature is set, and the resist- chamber, and press START. Ance increases until it reaches the target tem- perature. At “START Ready?”, press START. Cooling assembly, main control board, or application board. ® The Advanced 3D3/4D3 Service Manual. STOP twice to exit the decrease the amplitude. (The default is menu.

98, but your value may differ.) 3D3 The stir wire movement should be Block Bin Setting no wider than the width of the sam- ple probe. Press to select instrument. “Beeper Test”, and then press START and 4000, Out of The sample tested was beyond the range Dilute the sample and retest.

Range” (3D3 only) of the instrument. “Sample Did Not The sample did not freeze (after 1. Make sure the sample tube is not empty, Freeze, Please Repeat”. If problem persists, replace the processor “System Error: ID” board. “System Error: Unknown” “System Error: Requests” “System Error: Primitive” “System Error: Interrupt” “System Error: Communications” “Illegal Error Code: “Unknown Error” “Memory Allocation Error” “ROM Serial Number Error” ® The Advanced 3D3/4D3 Service Manual. Retest the sample in the low range setting. Range” ting was under 1400 mOsm.

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